Primer

Part:BBa_K5427016

Designed by: Brittany Green   Group: iGEM24_UAlberta   (2024-09-23)


FW pET22b+ Keratin

Forward primer for linearization of pET22b+ vector with keratin31.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 5
    Illegal PstI site found at 21
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 5
    Illegal PstI site found at 21
    Illegal NotI site found at 12
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 26
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 5
    Illegal PstI site found at 21
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 5
    Illegal PstI site found at 21
  • 1000
    COMPATIBLE WITH RFC[1000]


[edit]
Categories
Parameters
None